Objective  To formulate a three-level screening process for COVID-19 to cut off the virus transmis-sion route as early as possible and reduce the exposure to virus sources. Methods  The first-level screening consisted of two steps triage;  fast triage station in each entrance of medical areas and fever clinic triage station with forward  checklist screening to find  out suspected  patients  as early  as  possible. The  secondary  screening was  performed for patients in a completely closed management manner,  to further confirm the epidemiological history and symptoms,and then combine with blood routine,  chest radiograph or CT imaging results to confirm suspected cases.The tertiary screening focused on the management of suspected cases. The patients with COVID-19 were accuratelyscreened through two nucleic  acid tests.  Results   Until 8;00 AM  of 9th February,1589  suspected  patients were screened  in the triage  stations  of fever clinic  which  was first-level  screening. 282  cases  were  screened  in fever clinic  which was secondary  screening, 39 suspected patients were  screened in isolated  observation area which  was tertiary screening. In the end,  3 cases were confirmed and transported to the designed hospital for isolation treatment. No nurses and doctors were infected and no patients were missed. Conclusion The three-level screening process is clear and strictly prevents missed diagnosis,  and effectively reduces the risk of transmission within medical institutions.