Objective To explore the barriers and facilitators of evidence-based practice of comprehensive ICU nursing handoff，and provide basis for promoting clinical transformation of evidence. Methods Objective sampling method was used to select nursing managers and clinical nurses in comprehensive ICU ward of a tertiary grade A hospital in Zigong，Sichuan Province as research objects. The interview outline was constructed based on the consolidated framework for implementation research（CFIR），and 11 clinical nurses and nursing managers were interviewed by semi-structured interview method，and the interview contents were coded by directional content analysis method. Results A total of 6 barriers and 14 facilitators covering 4 fields of CFIR framework were obtained through interviews. There were 4 themes summarized. At the practitioner level，comprehensive ICU nurses had fully recognized the importance of handoff，and prioritized the evidence-based practice plan of handoff，but they lacked their own knowledge of evidence-based nursing. At the program level，the handoff evidence-based practice program was scientific and effective，but its complexity hindered its implementation. At the resource level，the implementation of the handoff evidence-based practice program needed manpower，time and information system support. In terms of organizational environment，department culture，atmosphere，and leadership promoted the implementation of evidence-based practice programs for transition. Conclusion This study identified the barriers and facilitators in the clinical transformation of evidence related to nursing handoff from the four levels of practitioners，program，available resources and organizational environment，and provided a basis for improving the clinical applicability of program and drafting implementation strategies.