Objective To compare the application effects of different sealing solutions in totally implantable venous access port among tumor patients. Methods A randomized sampling was used to select 414 patients who were maintained infusion port in outpatient department of a tertiary Grade A hospital in Zhejiang Province from December 2018 to December 2022. They were divided into three groups，A，B，and C，with 138 cases in each group，using a random number table method. Patients were treated with 5 ml 0.9% sodium chloride for positive pressure sealing during intermittent maintenance in group A，and 5 ml heparin（10 U/ml） for positive pressure sealing in group B，and 5ml heparin（100 U/ml） positive pressure sealing in group C. To observe coagulation function and catheter blockage in patients among three groups. Results There were statistically significant differences in prothrombin time and activated partial thrombin time among the three groups（P<0.05）. There was no significant difference in D-dimer quantity and platelet count（P>0.05）. The pair comparison of prothrombin time among the three groups showed that there was no statistical significance between group A and group B（t=-34.409，P>0.05），but there were statistically significant differences between group A and group C（t=-179.308，P<0.001） and between group B and group C（t=-144.899，P<0.001）. The pairwise comparison of activated partial thromboplastin time among the three groups showed that there was no statistical significance between group A and group B（t=1.406，P>0.05）， but there were statistically significant differences between group A and group C（t=-35.493，P<0.05） and between group B and group C（t=-36.899，P<0.05）. Conclusion Among cancer patients with totally implantable access port during intermittent maintenance period，the use of heparin solution（100 U/ml） can prolong prothrombin time andactivated partial thromboplastin time. It is recommended that clinical nursing staff seal the tube with 0.9% sodium chloride or heparin solution（10 U/ml） in outpatient patients undergoing intermittent maintenance.